The Design of a Quantitative Western Blot Experiment
نویسندگان
چکیده
منابع مشابه
The Design of a Quantitative Western Blot Experiment
Western blotting is a technique that has been in practice for more than three decades that began as a means of detecting a protein target in a complex sample. Although there have been significant advances in both the imaging and reagent technologies to improve sensitivity, dynamic range of detection, and the applicability of multiplexed target detection, the basic technique has remained essenti...
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Accurate and reproducible quantifi cation of proteins on Western blots across a wide linear range is desirable, but diffi cult to achieve. Chemiluminescent detection is subject to enzyme/substrate kinetics that may affect performance, and use of antibodies labeled with visible fl uorophores has not provided the necessary sensitivity for practical use. In this study, we investigated the use of a...
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ژورنال
عنوان ژورنال: BioMed Research International
سال: 2014
ISSN: 2314-6133,2314-6141
DOI: 10.1155/2014/361590