The cytokinesis-block micronucleus assay for cryopreserved whole blood
نویسندگان
چکیده
منابع مشابه
Testing chemical agents with the cytokinesis-block micronucleus cytome assay.
In order to evaluate the applicability of the cytokinesis-block micronucleus cytome assay in routine mutagenicity testing we investigated with this method different chemicals having different mechanisms of action: non-mutagens, direct-acting basealtering mutagens, direct-acting cross-linking mutagens, clastogens including a radiomimeti...
متن کاملLaboratory intercomparison of the cytokinesis-block micronucleus assay.
The focus of the study is an intercomparison of laboratories' dose-assessment performances using the cytokinesis-block micronucleus (CBMN) assay as a diagnostic triage tool for individual radiation dose assessment. Homogenously X-irradiated (240 kVp, 1 Gy/min) blood samples for establishing calibration data (0.25-5 Gy) as well as blind samples (0.1-6.4 Gy) were sent to the participants. The CBM...
متن کاملMicronucleus assay of human lymphocytes: a comparison of cytokinesis-block and human capillary blood lymphocytes methods.
Micronucleus (MN) expression in Peripheral blood lymphocytes is well established as a method to monitor chromosome damage in human populations. The use of MN as a measure of chromosome damage in peripheral blood lymphocytes was first proposed by Countryman and Heddle and subsequently improved with the development of the cytokinesis-block micronucleus (CBMN) method, which has been extensively us...
متن کاملThe use of the lymphocyte cytokinesis-block micronucleus assay for monitoring pesticide-exposed populations.
Pesticides are widely used around the world, and hundreds of millions of people are exposed annually in occupational and environmental settings. Numerous studies have demonstrated relationships between pesticide exposure and increased risk of cancers, neurodegenerative and neurodevelopmental disorders, respiratory diseases and diabetes. Assessment of genotoxicity of pesticides and biomonitoring...
متن کاملApplication of cell sorting for enhancing the performance of the cytokinesis-block micronucleus assay.
Among the numerous methods available to assess genotoxicity, the cytokinesis-block micronucleus (CBMN) assay is very popular due its relative simplicity and power to detect both clastogenic and aneugenic compounds. A problem with the CBMN assay is that all DNA damaging agents also inhibit the ability of cells to progress through mitosis, leading to a low number of binucleated cells (BNCs). One ...
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ژورنال
عنوان ژورنال: International Journal of Radiation Biology
سال: 2021
ISSN: 0955-3002,1362-3095
DOI: 10.1080/09553002.2021.1941378