The collagen substrate specificity of human neutrophil collagenase.
نویسندگان
چکیده
منابع مشابه
Substrate Specificity of &Collagenase from CZostridium histolyticum”
The substrate specificity of #?-collagenase from Clostridium histolyticum has been investigated by measuring the rate of hydrolysis of more than 50 tri-, tetra-, penta-, and hexapeptides covering the P3 to Ps’ subsites of the substrate. The choice of peptides was patterned after sequences found in the a1 and a2 chains of type I collagen. Each peptide contained either a 2-furanacryloyl (FA) or c...
متن کاملStructure-function relationship of human neutrophil collagenase: identification of regions responsible for substrate specificity and general proteinase activity.
The family of matrix metalloproteinases is a family of closely related enzymes that play an important role in physiological and pathological processes of matrix degradation. The most distinctive characteristic of interstitial collagenases (fibroblast and neutrophil collagenases) is their ability to cleave interstitial collagens at a single peptide bond; however, the precise region of the enzyme...
متن کاملSecreted Human Neutrophil Collagenase 5647
Collagenase in human neutrophils is found within intracellular granules which can be stimulated to be secreted with phorbol myristic acetate. This extracellular secreted form of neutrophil collagenase was isolated by immunoaffinity chromatography using a monoclonal antibody previously shown to specifically recognize neutrophil collagenase. The enzyme efficiently bound to this column and was ...
متن کاملHuman neutrophil collagenase cleaves x 1 - antitrypsin
Inactivation of the plasma serine-proteinase inhibitor a1-antitrypsin (a1-AT) by neutrophil metalloproteinases has been reported [Vissers, George, Bathurst, Brennan & Winterbourn (1987) Fed. Proc. Fed. Am. Soc. Exp. Biol. 46, 1390a; (1988) J. Clin. Invest. 82, 706-711; Desrochers & Weiss (1988) J. Clin: Invest. 81, 1646-1650]. To identify the enzyme responsible, supernatant from neutrophils sti...
متن کاملThe latent collagenase and gelatinase of human polymorphonuclear neutrophil leucocytes.
Two metallo-proteinases of human neutrophil leucocytes, collagenase and gelatinase, were studied. Collagenase specifically cleaved native collagen into the TCA and TCB fragments, whereas gelatinase degraded denatured collagen, i.e. gelatin, and the TCA fragments produced by collagenase. On subcellular fractionation by zonal sedimentation, collagenase was found to be localized in the specific gr...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1987
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)61073-7