The characterization of human oral mucosal fibroblasts and their use as feeder cells in cultivated epithelial sheets
نویسندگان
چکیده
منابع مشابه
The characterization of human oral mucosal fibroblasts and their use as feeder cells in cultivated epithelial sheets
Aim To characterize human oral mucosa middle interstitial tissue fibroblasts (hOMFs) and their application in the cultivation of epithelial sheets. Methodology hOMFs were cultured with methylcellulose to form cell clusters. hOMFs amplified in adhesive culture were analyzed by flow cytometry, and were found to differentiate into multiple cell types suitable for the cultivation of human corneal...
متن کاملFormation of epithelial sheets by serially cultivated human mucosal cells and their applications as a graft material.
A cultured epithelial sheet can be formed from living mucosal cells in vitro and used as a graft material. In this article, we describe our culturing methods for the preparation of mucosal epithelial sheets as well as the biological characteristics of these sheets compared with those of skin epithelial sheets. A cultured epithelial sheet has 5 to 8 cell layers and sufficient mechanical strength...
متن کاملcultivated limbal and oral mucosal epithelial transplantation
stem cells located at the limbus , are the ultimate source for regeneration of the corneal epithelium in the normal and traumatized states. when limbal stem cells are dysfunctional or deficient, limbal stem cell deficiency (lscd) develops. its surgical management depends on laterality and severity of corneal-limbal involvement. conventional methods of stem cell transplantation are conjunctiva...
متن کاملMolecular characterization of explant cultured human oral mucosal epithelial cells.
PURPOSE To culture and characterize oral mucosal epithelial cells (OMEC) grown on de-epithelialized human amniotic membrane (HAM) to explore their suitability as autografts in patients with bilateral ocular surface disease (OSD) and limbal stem cell deficiency. METHODS Oral biopsy samples were obtained from 20 patients undergoing oral reconstructive surgery, with informed consent and Institut...
متن کاملBarrier function of cultivated limbal and oral mucosal epithelial cell sheets.
PURPOSE To investigate barrier function of cultivated limbal and oral mucosal epithelial sheets. METHODS Human and rabbit cultivated limbal epithelial sheets (CLES) and cultivated oral mucosal epithelial sheets (COMES) were subjected to immunohistochemistry for keratin K3, K4, K13, ZO-1, and occludin. Results were compared with those obtained from normal cornea and oral mucosa. Prepared under...
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ژورنال
عنوان ژورنال: Future Science OA
سال: 2017
ISSN: 2056-5623
DOI: 10.4155/fsoa-2017-0074