The cap and the 3' splice site similarly affect polyadenylation efficiency
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چکیده
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the relationship between learners critical thinking ability and their performance in the reading sections of the tofel and ielts test
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15 صفحه اولA nuclear cap-binding complex facilitates association of U1 snRNP with the cap-proximal 5' splice site.
The mechanism by which intron-containing RNAs are recognized by the splicing machinery is only partly understood. A nuclear cap-binding complex (CBC), which specifically recognizes the monomethyl guanosine cap structure carried by RNA polymerase II transcripts, has previously been shown to play a role in pre-mRNA splicing. Using a combination of splicing complex and psoralen cross-linking analy...
متن کاملThe organization of 3' splice-site sequences in mammalian introns.
A model pre-mRNA substrate was used to carry out a detailed investigation of the functional organization of sequences at the 3' end of mammalian introns. This analysis revealed a difference in the sequence requirements for the first and second steps of the splicing reaction (lariat formation and exon ligation, respectively). Maximal efficiencies of lariat formation require a pyrimidine stretch ...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1996
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.16.6.2579