منابع مشابه
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes.
The Berkeley Drosophila Genome Project (BDGP) strives to disrupt each Drosophila gene by the insertion of a single transposable element. As part of this effort, transposons in >30,000 fly strains were localized and analyzed relative to predicted Drosophila gene structures. Approximately 6300 lines that maximize genomic coverage were selected to be sent to the Bloomington Stock Center for public...
متن کاملGene disruption in Salmonella typhimurim by modified λ Red disruption system
There are many techniques to knock out directed genes in bacteria, some of which have been described in Salmonella species. In this study, a combination of SOEing PCR method and the λ Red disruption system were used to disrupt phoP gene in wild type and standard strains of Salmonella typhimurium. Three standards PCR and one fusion PCR reactions were performed to construct a linear DNA including...
متن کاملThe Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes.
A fundamental goal of genetics and functional genomics is to identify and mutate every gene in model organisms such as Drosophila melanogaster. The Berkeley Drosophila Genome Project (BDGP) gene disruption project generates single P-element insertion strains that each mutate unique genomic open reading frames. Such strains strongly facilitate further genetic and molecular studies of the disrupt...
متن کاملThe Drosophila gene disruption project: progress using transposons with distinctive site specificities.
The Drosophila Gene Disruption Project (GDP) has created a public collection of mutant strains containing single transposon insertions associated with different genes. These strains often disrupt gene function directly, allow production of new alleles, and have many other applications for analyzing gene function. Here we describe the addition of ∼7600 new strains, which were selected from >140,...
متن کاملDisruption management for resource-constrained project scheduling
In this paper, we study the problem of how to react when an ongoing project is disrupted. The focus is on the resourceconstrained project scheduling problem with finish–start precedence constraints. We begin by proposing a classification scheme for the different types of disruptions and then define the constraints and objectives that comprise what we call the recovery problem. The goal is to ge...
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ژورنال
عنوان ژورنال: Genetics
سال: 2004
ISSN: 1943-2631
DOI: 10.1534/genetics.104.026427