The antioxidant, anti-angiogenic, and anticancer impact of chitosan-coated herniarin-graphene oxide nanoparticles (CHG-NPs)

BackgroundHerniarin, a simple coumarin found in chamomile leaf rosettes is known as the oxidative stress protector. In current study, herniarin was captured into Graphene oxide nanoparticles and coated with chitosan poly-cationic polymer to be used novel bio-compatible nano-drug delivery system investigate its antioxidant, anti-angiogenic anti-cancer impacts on human lung A549 cancer cells.MethodThe Chitosan-coated Herniarin-Graphene (CHG-NPs) were designed, produced, characterized utilizing DLS, FESEM, FTIR, Zeta-potential analysis. The CHG-NPs’ antioxidant activity analyzed by conducting ABTS DPPH assays. CAM assay verified measuring VEGF VEGFR gene expression levels following their increased treatment doses applying Q-PCR technique. Finally, cytotoxicity studied cells.ResultThe stable (+27.11 mV) 213.6-nm CHG-NPs significantly inhibited ABTS/DPPH free radicals exhibited activity. suppressed angiogenesis process vessels observed detecting decreased length/number of vessels. Moreover, down-regulated blood nanoparticles’ potential. selective cytotoxic impact cells compared normal HFF cell line.ConclusionThe cytotoxicity, strong activity, significant property nano-scaled produced make it an appropriate anticancer system. Therefore, have potential anti-lung compound.