The antioxidant activity of lemongrass leaves extract against fibroblasts oxidative stress

Objective: The purpose of this research is to assess the antioxidant activity lemongrass leaves extract in terms lowering ROS generation and its effect on viability proliferation fibroblasts under oxidative stress. Material Methods: was measured using DPPH method assay carried out by fluorescent H2DCFDA staining. Viability assays were performed Cell Counting Kit-8 (CCK-8) read at 450 nm microplate reader. groups divided into 8, namely without treatment (comparison group), fibroblast induced H2 O2 (negative control), with then treated ascorbic acid (positive various concentrations (10, 20, 30, 40, 50 ppm). Results: results showed that had an IC50 value 64.17 ppm. production reduced LgLE all if compared negative control (p=0.819). can maintained 10 ppm most optimum concentration (p<0.05). fibroblast, effective 24 h observation (p><0.05). . Conclusion: Lemongrass has a strong reduce stress increase KEYWORDS Antioxidant activity; Fibroblast; Lemongrass; Oxidative stress; Reactive oxygen species.