The 3'-terminal nucleotide sequences of adenovirus types 2 and 5 DNA

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The 3'-terminal nucleotide sequences of adenovirus types 2 and 5 DNA.

Short nucleotide sequences at the 3'-termini of adenovirus types 2 and 5 DNA have been determined using T4 DNA polymerase as described by P. T. Englund (1972). The terminal sequences of both serotypes appear to be completely identical. Both molecular ends of type 2 as well as of type 5 DNA terminate with the sequence ...pCpC...pGpApTpG3', consistent with the presence of an inverted terminal rep...

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The sequence of 5,839 nucleotides from the adenovirus 2 genome has been determined and includes the regions between coordinates 32-44% and 66-71%. These regions contain the coding sequences for the 52,55K polypeptide, polypeptide IIIa, penton base, and the N terminus of the 100K polypeptide. Several additional unidentified open reading frames are present, including examples which overlap identi...

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The length of the terminal repetition in adenovirus-2 DNA.

Adenovirus-2 DNA was end-labeled by partial digestion with Escherichia coli exonuclease III and resynthesis with the DNA polymerase from avian myeloblastosis virus and alpha-(32)P-labeled deoxyribonucleoside triphosphates. This end-labeled DNA was cleaved with several specific endonucleases and the terminal fragments were characterized by gel electrophoresis and pyrimidine tract analysis. Two e...

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15 صفحه اول

Analysis of the 5'-terminal nucleotide sequences of ribonucleic acids. II. Comparison of the 5'-terminal nucleotide sequences of ribosomal RNA's from different organisms.

The use of end-group analysis is valuable for determining the homogeneity and molecular weight of macromolecules. The synthesis of RNA upon a DNA template proceeds from the 5'to the 3'-end of the RNA sequence, and the identification of the 5'-termini of RNA molecules is therefore of particular importance in characterizing them. With this in view, a procedure for analyzing 5'-terminal nucleotide...

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 1975

ISSN: 0022-538X,1098-5514

DOI: 10.1128/jvi.15.2.268-272.1975