Th1 / Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows

The aim of this study to determine the Th1/Th2 cytokine balance in milk according bacterial species that cause subclinical mastitis cows. California Mastitis Test (CMT) was applied selected cows were divided into four groups: with negative CMT (n = 45); Escherichia coli (E. coli) group included only E. growing CMT-positive samples Streptococcus agalactiae (S. agalactiae) S. Staphylococcus aureus aureus) 45). Somatic cell count (SCC) fresh measured using DeLaval Cell Counter device. Also, analyses performed Species-specific commercial ELISA kits. Tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) concentrations relatively high group, but interleukin (IL)-2 concentration low. lowest IL-4 found CMT-negative group. highest IL-5 while IL-10 T helper (Th1/Th2) polarization shifted towards Th1 caused by coli. Th2 agalactiae. Based on our findings, cellular immunity should be maintained cases due coli, humoral supported