Tetra Primer ARMS PCR Optimization to Detect Single Nucleotide Polymorphism of the KLF14 Gene
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چکیده
منابع مشابه
Detection of Single-Nucleotide Polymorphism in the Bovine AGPAT6 Gene Associated with Milk Fat Content using Tetra-Primer ARMS PCR-based Assay in Karan Fries Breeding Bulls
Background: The bovine AGPAT6 gene is one of the potential candidate genes governing milk fat synthesis.Objectives: Identification of single nucleotide polymorphisms (SNP) in the targeted region of AGPAT6 gene and their effect on expected breeding values (EBV) of first lactation milk production traits viz. fat %, fat yield and 305 days milk yield in Karan Fries (KF) breeding bulls were so...
متن کاملGenotyping single nucleotide polymorphisms in barley by tetra-primer ARMS-PCR.
Single nucleotide polymorphisms (SNPs) are the most abundant form of DNA polymorphism. These polymorphisms can be used in plants as simple genetic markers for many breeding applications, for population studies, and for germplasm fingerprinting. The great increase in the available DNA sequences in the databases has made it possible to identify SNPs by "database mining", and the single most impor...
متن کاملtetra-primer arms pcr and its applications in genotyping point mutations and single nucleotide polymorphisms
abstract
متن کاملPrimer1: Primer Design Web Service for Tetra-Primer ARMS-PCR
Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given...
متن کاملA Novel Multiplex Tetra-Primer ARMS-PCR for the Simultaneous Genotyping of Six Single Nucleotide Polymorphisms Associated with Female Cancers
BACKGROUND The tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) is a fast and economical means of assaying SNP's, requiring only PCR amplification and subsequent electrophoresis for the determination of genotypes. To improve the throughput and efficiency of T-ARMS-PCR, we combined T-ARMS-PCR with a chimeric primer-based temperature switch PCR (TSP) strategy, and used capil...
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ژورنال
عنوان ژورنال: OALib
سال: 2017
ISSN: 2333-9721,2333-9705
DOI: 10.4236/oalib.1104145