Testing reliability of a potential island mating apiary using DNA microsatellites

Reliability of an island mating apiary under routine management

Introduction Islands are widely used to achieve controlled matings of virgin queens because queens and drones are not expected to cross open water during mating flights. However, uncontrolled matings on island mating areas have repeatedly been reported for many islands in close proximity to mainland shorelines (c.f. Neumann et al., 1999b). With increasing distance between island and mainland on...

DNA fingerprinting of bluegill sunfish (Lepomis macrochirus) using (GT)n microsatellites and its potential for assessment of mating success

Variable microsatellite DNA markers are powerful tools to study parentage and gene flow in fish populations. In this work, we cloned and characterized 60 (GT) repeat microsatellite sequences from the bluegill sunfish (Lepomis macrochirus) genome. We report the frequency distribution, the lengths, and the proportions of the repeat classes for comparison with microsatellites isolated from other t...

Determining Component Reliability Using a Testing Index

Software reliability assessment using accelerated testing methods

The use of operational pro®les and usage-based testing has received considerable attention recently in the software engineering literature. Testing under the actual operational pro®le can, however, be expensive, time consuming or even infeasible in situations where the performance of a system is dominated by infrequent but highly critical events. We consider a real application that deals with t...

Parentage Verification of Iranian Caspian Horse Using Microsatellites Markers

The present study was to construct a parentage verification system for Iranian Caspian horse. A total number of 45 Caspian horse samples including 14 foals for parentage verification, 17 stallion and 14 mare for individual identification were genotyped. Genomic DNA was extracted from whole blood and the genotype were analysed by PCR procedure and using 7 microsatellite markers (AHT04, HMS03, HM...