Termination of DNA synthesis by N6 -alkylated, not 3′- O -alkylated, photocleavable 2′-deoxyadenosine triphosphates
نویسندگان
چکیده
منابع مشابه
Termination of DNA synthesis by N6-alkylated, not 3′-O-alkylated, photocleavable 2′-deoxyadenosine triphosphates
The Human Genome Project has facilitated the sequencing of many species, yet the current Sanger method is too expensive, labor intensive and time consuming to accomplish medical resequencing of human genomes en masse. Of the 'next-generation' technologies, cyclic reversible termination (CRT) is a promising method with the goal of producing accurate sequence information at a fraction of the cost...
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We describe a novel 3'-OH unblocked reversible terminator with the potential to improve accuracy and read-lengths in next-generation sequencing (NGS) technologies. This terminator is based on 5-hydroxymethyl-2'-deoxyuridine triphosphate (HOMedUTP), a hypermodified nucleotide found naturally in the genomes of numerous bacteriophages and lower eukaryotes. A series of 5-(2-nitrobenzyloxy)methyl-dU...
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The phototriggered cleavage of chemical bonds has found numerous applications in biology, particularly in the field of gene sequencing through photoinduced DNA strand scission. However, only a small number of modified nucleosides that are able to cleave DNA at selected positions have been reported in the literature. Herein, we show that a new photoactivable deoxyadenosine analogue, 3-nitro-3-de...
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C-13-alkylated methoxy-8tf-dibenzo[a,g]quinolizin-8-ones 2a-e were synthesized by photocyclization of 1 -alkylidene-N-benzoyl-1,2,3,4-tetrahydroisoquinolines 1. Moreover, condensation of l,2,3,4-tetrahydro-6,7-dimethoxy-l-oxo-isoquinoline with homophthalic acid anhydrides 7a and b leads to the C-13-alkylated 8-oxoberbines 2b and c and improves the yields compared with those of the photocyclizat...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2007
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkm689