Terminal deoxynucleotidyl transferase in human lymphomas: possible existence of forms with high and low molecular weights
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چکیده
منابع مشابه
Purification of a high molecular weight human terminal deoxynucleotidyl transferase.
Terminal deoxynucleotidyltransferase has been purified from lymphoblasts of leukemic patients. The enzyme has a molecular weight of approximately 62,000 as determined by gel filtration and nondenaturing gel electrophoresis and is not dissociated into subunits by sodium dodecyl sulfate. In contrast, the terminal transferase enzyme from calf thymus has a molecular weight of 42,000 as determined b...
متن کاملMurine Terminal Deoxynucleotidyl Transferase
Terminal deoxynucleotidyl transferase (TdT) 1 is an enzyme which has the unique property of polymerizing deoxyribonucleotides onto a primer in the absence of a template (1-4) . Chang (4) first reported that TdT is present only in the thymus among various avian and mammalian tissues examined . Her finding was extended to humans by McCaffrey et al . (5), who found that the enzyme was restricted t...
متن کاملAssociation of terminal deoxynucleotidyl transferase with Ku.
Terminal deoxynucleotidyl transferase (TdT) catalyzes the addition of nucleotides at the junctions of rearranging Ig and T cell receptor gene segments, thereby generating antigen receptor diversity. Ku is a heterodimeric protein composed of 70- and 86-kDa subunits that binds DNA ends and is required for V(D)J recombination and DNA double-strand break (DSB) repair. We provide evidence for a dire...
متن کاملDetection of terminal deoxynucleotidyl transferase.
The recently published study of peripheral blood lymphocyte subpopulations in patients with acute immunodeficiency syndrome, by Murray et al,’ may have contributed additional insight into normal T cell ontogenesis. However, the data on terminal transferase (TdT) must be interpreted with caution. It is stated that TdT levels are especially elevated in acute lymphocytic leukemia (ALL) of T cell o...
متن کاملHigh Terminal Deoxynucleotidyl Transferase Activity in Acute Myelogenous Leukemia1
Although leukocytes from all 13 acute lymphoblastic ieu kemia patients examined had high terminal deoxynucleoti dyl transferase (terminal transferase) activity (20 to 100 units/mg of cellular DNA, where 1 unit equals 1 nmole of nucleotide polymenized in 1 hr) and those from 21 acute myelocytic leukemia patients had low terminal transferase activity (0.2 to 2 units/mg of cellular DNA), the bone ...
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ژورنال
عنوان ژورنال: British Journal of Cancer
سال: 1981
ISSN: 0007-0920,1532-1827
DOI: 10.1038/bjc.1981.49