Temporal Assessment of Biofumigation Using Mustard and Oilseed Rape Tissues on Verticillium dahliae, Soil Microbiome and Yield of Eggplant

Verticillium dahliae is a soil-borne pathogen that difficult to eliminate, causes severe reduction in the yield and quality of eggplant, could be controlled through environmentally friendly biofumigation. However, mechanisms its effects on dynamic changes soil microbes not clear. In this study, we examined efficacy two Brassica vegetables, mustard (Brassica napiformis, BFN) oilseed rape napus, BFC), as biofumigants control observed their microbial community compared with chemical fumigants (CF) (CN) greenhouse where eggplant was continuously cultivated for 12 years. Illumina MiSeq sequencing used analyse microbiome. Real-time PCR assay order estimate abundance dahlia, glucosinolate concentration determined using HPLC. Results showed BFN treatment had significant biocidal effect V. soil, decreasing by 64.74% (CN). No difference between BFC CN treatments. Yield improved due lower disease incidence rate index. Biofumigation structure bacteria fungi. The bacterial diversity significantly than those other treatments, beneficial relative abundance, such Flavobacteria, Pseudomonas Bacillus, increased after biofumigated 15 days. temporal fungi among different treatments reduced development growth stage. Mortierella, Altemaria Chaetomium genera were abundant across all Soil enzyme activities also improved. summary, biofumigation efficient controlling wilt composition at early stage; initial conditions are key determinant what going happen biofumigation, which considered practical addition integrated pest management pathogens.