Tempol differently affects cellular redox changes and antioxidant enzymes in various lung-related cells

Abstract Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl) is a potential redox agent in cells. The present study investigated changes cellular reactive oxygen species (ROS) and glutathione (GSH) levels antioxidant enzymes, Tempol-treated Calu-6 A549 lung cancer cells, normal WI-38 VA-13 primary pulmonary fibroblasts. Results demonstrated that (0.5–4 mM) either increased or decreased general ROS cells at 48 h specifically O 2 •− these In addition, differentially altered the expression activity of enzymes such as superoxide dismutase, catalase, thioredoxin reductase1 (TrxR1) A549, Calu-6, particular, treatment TrxR1 protein 1 mM inhibited growth by about 50% but also significantly induced cell death, evidenced annexin V-positive Furthermore, down-regulation siRNA had some effect on well inhibition death -untreated doses numbers GSH-depleted both h. conclusion, various all along with an increase GSH depletion.