Telomere Dysfunction Drives Increased Mutation by Error-Prone Polymerases Rev1 and ζ in Saccharomyces cerevisiae
نویسندگان
چکیده
منابع مشابه
Telomere dysfunction drives increased mutation by error-prone polymerases Rev1 and zeta in Saccharomyces cerevisiae.
Using a model system, we have shown that replicative senescence is accompanied by a 16-fold increase in base substitution and frameshift mutations near a chromosome end. The increase was dependent on error-prone polymerases required for the mutagenic response to DNA lesions that block the replication fork.
متن کاملDNA polymerases ζ and Rev1 mediate error-prone bypass of non-B DNA structures
DNA polymerase ζ (Pol ζ) and Rev1 are key players in translesion DNA synthesis. The error-prone Pol ζ can also participate in replication of undamaged DNA when the normal replisome is impaired. Here we define the nature of the replication disturbances that trigger the recruitment of error-prone polymerases in the absence of DNA damage and describe the specific roles of Rev1 and Pol ζ in handlin...
متن کاملDNA polymerases f and Rev1 mediate error-prone bypass of non-B DNA structures
DNA polymerase f (Pol f) and Rev1 are key players in translesion DNA synthesis. The error-prone Pol f can also participate in replication of undamaged DNA when the normal replisome is impaired. Here we define the nature of the replication disturbances that trigger the recruitment of error-prone polymerases in the absence of DNA damage and describe the specific roles of Rev1 and Pol f in handlin...
متن کاملThe DNA polymerase activity of Saccharomyces cerevisiae Rev1 is biologically significant.
A cell's ability to tolerate DNA damage is directly connected to the human development of diseases and cancer. To better understand the processes underlying mutagenesis, we studied the cell's reliance on the potentially error-prone translesion synthesis (TLS), and an error-free, template-switching pathway in Saccharomyces cerevisiae. The primary proteins mediating S. cerevisiae TLS are three DN...
متن کاملDNA Polymerase ζ-Dependent Lesion Bypass in Saccharomyces cerevisiae Is Accompanied by Error-Prone Copying of Long Stretches of Adjacent DNA
Translesion synthesis (TLS) helps cells to accomplish chromosomal replication in the presence of unrepaired DNA lesions. In eukaryotes, the bypass of most lesions involves a nucleotide insertion opposite the lesion by either a replicative or a specialized DNA polymerase, followed by extension of the resulting distorted primer terminus by DNA polymerase ζ (Polζ). The subsequent events leading to...
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ژورنال
عنوان ژورنال: Genetics
سال: 2007
ISSN: 1943-2631
DOI: 10.1534/genetics.106.068130