Technique for the Isolation of Ribonucleic Acid-Rich Mutants from Escherichia coli
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چکیده
منابع مشابه
Technique for the isolation of ribonucleic acid-rich mutants from Escherichia coli.
A technique for the isolation of ribonucleic acid (RNA)-rich mutants of Escherichia coli is described. Mutagenized cells were centrifuged to isopycnic equilibrium on potassium tartrate or cesium sulfate gradients. Samples from the region of the gradients slightly denser than the majority of the cells were spread on agar plates, and the resulting clones were tested for increased RNA to protein r...
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E. coli S-RNA’ was obtained from General Biochemicals. Other preparations were available in this laboratory. A sample of S-RNA prepared from Salmonella typhimurium was the gift of Dr. David Smith. The 4-thiouridine disulfide was a gift of Dr. J. Fox, and another portion was obtained from Cycle Chemical Company. Phosphomonoesterase from E. coli was kindly provided by Dr. Leon Heppel, and samples...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1969
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.100.2.974-976.1969