Technique for quantitative RT-PCR analysis directly from single muscle fibers
نویسندگان
چکیده
منابع مشابه
Technique for quantitative RT-PCR analysis directly from single muscle fibers.
The use of single-cell quantitative RT-PCR has greatly aided the study of gene expression in fields such as muscle physiology. For this study, we hypothesized that single muscle fibers from a biopsy can be placed directly into the reverse transcription buffer and that gene expression data can be obtained without having to first extract the RNA. To test this hypothesis, biopsies were taken from ...
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ژورنال
عنوان ژورنال: Journal of Applied Physiology
سال: 2008
ISSN: 8750-7587,1522-1601
DOI: 10.1152/japplphysiol.00897.2007