TBP, a universal eukaryotic transcription factor?

TBP, a universal eukaryotic transcription factor?

Duality of TBP, the universal transcription factor.

Transcription factor IIA derepresses TATA-binding protein (TBP)-associated factor inhibition of TBP-DNA binding.

The interaction of the general transcription factor (TF) IIA with TFIID is required for transcription activation in vitro. TFIID consists of the TATA-binding protein (TBP) and TBP associated factors (TAFIIs). TFIIA binds directly to TBP and stabilizes its interaction with TATA-containing DNA. In this work, we present evidence that TAFIIs inhibit TBP-DNA and TBP-TFIIA binding, and that TFIIA sti...

Crystal structure of RPB5, a universal eukaryotic RNA polymerase subunit and transcription factor interaction target.

Eukaryotic nuclei contain three different types of RNA polymerases (RNAPs), each consisting of 12-18 different subunits. The evolutionarily highly conserved RNAP subunit RPB5 is shared by all three enzymes and therefore represents a key structural/functional component of all eukaryotic RNAPs. Here we present the crystal structure of the RPB5 subunit from Saccharomyces cerevisiae. The bipartite ...

Their Physiological Roles Factor Specific Role General Transcription Factors ( GTFs ) a TBP Binds

cally changed. We now know that heterochromatin is Activators, chromatin-modifying enzymes, and basal rich in repetitive sequences but is not at all devoid of transcription factors unite to activate genes, but are genes. Moreover, euchromatic regions of chromosomes recruited in a precise order to promoters. The timing can be silenced by packaging in a heterochromatic form; of the activation of ...