منابع مشابه
Design, simplified cloning, and in-silico analysis of multisite small interfering RNA-targeting cassettes
Multiple gene silencing is being required to target and tangle metabolic pathways in eukaryotes and researchers have to develop a subtle method for construction of RNA interference (RNAi) cassettes. Although, several vectors have been developed due to different screening and cloning strategies but still some potential limitations remain to be dissolved. Here, we worked out a simple cloning stra...
متن کاملRNA-dependent RNA targeting by CRISPR-Cas9
Double-stranded DNA (dsDNA) binding and cleavage by Cas9 is a hallmark of type II CRISPR-Cas bacterial adaptive immunity. All known Cas9 enzymes are thought to recognize DNA exclusively as a natural substrate, providing protection against DNA phage and plasmids. Here, we show that Cas9 enzymes from both subtypes II-A and II-C can recognize and cleave single-stranded RNA (ssRNA) by an RNA-guided...
متن کاملTargeting RNA with small molecules.
5.1. Binding Sites of the Aminoglycosides 1185 5.2. Importance of Electrostatic Interactions 1185 5.3. Nonionic Interactions 1185 5.4. Pseudo-Base Pair Interactions 1186 5.5. Water-Mediated Contacts 1186 5.6. Shape Complementarity and Conformational Adaptation 1186 6. Assays for Evaluating RNA Binding 1186 6.1. Methods Utilizing Fluorescently Labeled RNA 1186 6.2. NMR-Based Methods 1187 6.3. El...
متن کاملSmall molecules targeting viral RNA.
Highly conserved noncoding RNA (ncRNA) elements in viral genomes and transcripts offer new opportunities to expand the repertoire of drug targets for the development of antiinfective therapy. Ligands binding to ncRNA architectures are able to affect interactions, structural stability or conformational changes and thereby block processes essential for viral replication. Proof of concept for targ...
متن کاملRNA-Targeting Therapies: Translating Hope
On July 20, 2016, the UK health regulator NICE published final guidexcitement for read-through therapies in some camps. An alternative apance recommending the drug ataluren for children with Duchenne muscular dystrophy (DMD). This was a momentous announcement for those who had campaigned vociferously for its approval in the UK. DMD is a rare X-linked disorder that causes progressive muscular we...
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ژورنال
عنوان ژورنال: Science-Business eXchange
سال: 2009
ISSN: 1945-3477
DOI: 10.1038/scibx.2009.1167