Targeted disruption of hir1 alters the transcriptional expression pattern of putative lignocellulolytic genes in the white-rot fungus Pleurotus ostreatus

Pleurotus ostreatus is frequently used in molecular genetics and genomic studies on white-rot fungi because various genetic tools relatively well-annotated genome databases are available. To explore the mechanisms underlying wood lignin degradation by P. ostreatus, we performed mutational analysis of a newly isolated mutant UVRM28 that exhibits decreased lignin-degrading ability beech sawdust medium. We identified mutation hir1 gene encoding putative histone chaperone, which probably plays an important role DNA replication-independent nucleosome assembly, responsible for phenotype. The expression pattern ligninolytic genes was altered disruptants. most highly expressed vp2 significantly inactivated, whereas vp1 remarkably upregulated (300–400 fold) at transcription level. Conversely, many cellulolytic xylanolytic were Chromatin immunoprecipitation suggested modification status 5ʹ-upstream regions some up- down-regulated lignocellulolytic disruptants compared with 20b strain. Hence, our data provide new insights into regulatory ostreatus.