Tannin extracted from Penthorum chinense Pursh, a potential drug with antimicrobial and antibiofilm effects against methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus

Staphylococcus aureus is an opportunistic pathogen. Due to the widespread use and abuse of antibiotics, various drug-resistant strains S. have emerged, with methicillin-resistant (MRSA) being most prevalent. Bacterial biofilm a significant contributor bacterial infection drug resistance. Consequently, formation has emerged as therapeutic strategy. In this study, chemical constituents, antimicrobial antibiofilm properties tannins isolated from Penthorum chinense Pursh (TPCP) were investigated. vitro , TPCP exhibited properties. The minimum inhibitory concentrations (MIC) bactericidal (MBC) for methicillin-sensitive (MSSA) MRSA 156.25 312.5 μg/mL, 625 respectively. According growth curves, significantly inhibited MSSA MRSA. results crystal violet assay in conjunction confocal laser scanning electron microscopy demonstrated that destroyed preformed biofilms. decreased secretion exopolysaccharides extracellular DNA. Subsequently, mechanism was investigated using RT-PCR. Examining expression ica A, cid sig B, agr sar A genes MRSA, we discovered by affecting quorum-sensing system bacteria. Our study demonstrates exerts antibacterial effects disrupting biofilms, suggesting clinical potential novel agent prevention treatment infections.