Tag/anti-tag liquid-phase primer extension array: A flexible and versatile genotyping platform

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Tag/anti-tag liquid-phase primer extension array: a flexible and versatile genotyping platform.

This study demonstrates an array-based platform to genotype simultaneously single nucleotide polymorphisms (SNPs) and some short insertions/deletions (indels) by the integration of the universal tag/anti-tag (TAT) system, liquid-phase primer extension (LIPEX), and a novel two-color detection strategy on an array format (TATLIPEXA). The TAT system permits a universal chip to be used for many app...

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Primer Design and Marker Clustering for Multiplex SNP-IT Primer Extension Genotyping Assay using Statistical Modeling

MOTIVATION The optimization of the primer design is critical for the development of high-throughput SNP genotyping methods. Recently developed statistical models of the SNP-IT primer extension genotyping reaction allow further improvement of primer quality for the assay. RESULTS Here we describe how the statistical models can be used to improve primer design for the assay. We also show how to...

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ژورنال

عنوان ژورنال: Genomics

سال: 2006

ISSN: 0888-7543

DOI: 10.1016/j.ygeno.2005.09.009