T7 Early RNAs are Generated by Site-Specific Cleavages

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T 7 Early RNAs are Generated by Site - Specific Cleavages ( E . coli RNA polymerase / initiation / termination / RNase III / rho factor )

Transcription of 17 DNA by purified Escherichia coli RNA polymerase without added factors produces long RNA molecules that begin near the left end of T7 DNA and terminate at the end of the early region. An endonuclease has been isolated from uninfected E. coli that cleaves these long RNAs at five specific sites to generate RNA molecules essentially the same as the early T7 RNAs observed in vivo...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1973

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.70.5.1559