Synthesis of rhamnolipid biosurfactant and mode of hexadecane uptake by Pseudomonas species
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منابع مشابه
Synthesis of rhamnolipid biosurfactant and mode of hexadecane uptake by Pseudomonas species
BACKGROUND Microorganisms have devised ways by which they increase the bioavailability of many water immiscible substrates whose degradation rates are limited by their low water solubility. Hexadecane is one such water immiscible hydrocarbon substrate which forms an important constituent of oil. One major mechanism employed by hydrocarbon degrading organisms to utilize such substrates is the pr...
متن کاملPseudomonas Rhamnolipid Surfactant (Biosurfactant)
A microbial surfactant (biosurfactant) was investigated for its potential to enhance bioavailability and, hence, the biodegradation of octadecane. The rhamnolipid biosurfactant used in this study was extracted from culture supernatants after growth of Pseudomonas aeruginosa ATCC 9027 in phosphate-limited proteose peptone-glucose-ammonium salts medium. Dispersion of octadecane in aqueous solutio...
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The objective of this research was to evaluate the effect of low concentrations of a rhamnolipid biosurfactant on the in situ biodegradation of hydrocarbon entrapped in a porous matrix. Experiments were performed with sand-packed columns under saturated flow conditions with hexadecane as a model hydrocarbon. Application of biosurfactant concentrations greater than the CMC (the concentration at ...
متن کاملPRODUCTION OF RHAMNOLIPID BIOSURFACTANT FROM A MARINE Pseudomonas aeruginosa
Biosurfactants are surface-active agents produced by hydrocarbanoclastic bacteria isolated from oil contaminated sites. In the present study a potential oil degrading bacterium was isolated from Cuddalore harbor waters and identified as Pseudomonas aeruginosa by using both biochemical and 16S rRNA sequencing. Growth optimization of the strain was done against varying physiochemical parameters v...
متن کاملIsolation and characterization of a regulatory gene affecting rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa.
A mutant strain (65E12) of Pseudomonas aeruginosa that is unable to produce rhamnolipid biosurfactants and lacks rhamnosyltransferase activity was genetically complemented by using a P. aeruginosa PG201 wild-type gene library. A single complementing cosmid was isolated on the basis of surface tension measurements of subcultures of the transconjugants by using a sib selection strategy. The subcl...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2009
ISSN: 1475-2859
DOI: 10.1186/1475-2859-8-16