Synthesis of Pyrimidine Deoxyribonucleoside Diphosphates with Enzymes from Escherichia coli
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چکیده
منابع مشابه
An Escherichia coli system expressing human deoxyribonucleoside salvage enzymes for evaluation of potential antiproliferative nucleoside analogs.
Deoxyribonucleoside salvage in animal cells is mainly dependent on two cytosolic enzymes, thymidine kinase (TK1) and deoxycytidine kinase (dCK), while Escherichia coli expresses only one type of deoxynucleoside kinase, i.e., TK. A bacterial whole-cell system based on genetically modified E. coli was developed in which the relevant bacterial deoxypyrimidine metabolic enzymes were mutated, and th...
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We examined the activity of polynucleotide phosphorylase (PNPase) from Streptomyces coelicolor, Streptomyces antibioticus, and Escherichia coli in phosphorolysis using substrates derived from the rpsO-pnp operon of S. coelicolor. The Streptomyces and E. coli enzymes were both able to digest a substrate with a 3' single-stranded tail although E. coli PNPase was more effective in digesting this s...
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D-Nucleoside analogs, which are in the natural configuration, as well as the L-nucleoside analogs, are clinically relevant antiviral and anticancer agents. Metabolism of L-nucleoside analog diphosphates to the triphosphates, however, remains unexplored. Studies with recombinant nm23-H1 and -H2 isoforms indicated that L-nucleoside analog diphosphates were not phosphorylated by their nucleoside d...
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Taylor, W. Herman (Portland State College, Portland, Ore.), and Mary L. Taylor. Enzymes of the pyrimidine pathway in Escherichia coli. II. Intracellular localization and properties of dihydroorotic dehydrogenase. J. Bacteriol. 88:105-111. 1964.-Intracellular localization of three enzymes of the pyrimidine pathway in Escherichia coli was studied. Dihydroorotic dehydrogenase was found to be assoc...
متن کاملEnzymatic synthesis of deoxyribonucleotides. I. Formation of deoxycytidine diphosphate from cytidine diphosphate with enzymes from Escherichia coli.
Extracts from Escherichiu coli catalyze the formation of deoxycyticlme phosphates from cytidine 5’-phosphate (1). Optimal formation of deoxynucleotides required the addition of adenosine triphosphate and Mg* ions to the extract, and, after treatment with Dowex 2, a strong stimulation of the reaction by reduced triphosphopyridine nucleotide was observed. Similar results were obtained in extracts...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1961
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)61744-8