Synthesis of fluorescent probe – carbohydrate conjugates
نویسندگان
چکیده
منابع مشابه
Ready protease-catalyzed synthesis of carbohydrate-amino acid conjugates.
The protease-catalyzed synthesis of amino acid est-carbohydrate conjugates as glycopeptide analogues has been achieved in a highly regioselective and carbohydrate-specific manner using amino acid vinyl ester acyl donors and minimally or completely unprotected carbohydrate acyl acceptors, which together probed active sites of proteases to reveal yield efficiencies that are modulated by the carbo...
متن کاملSynthesis and Characterization of a Mg2+-Selective Fluorescent Probe
A new Mg2+-selective fluorescent probe P was synthesized and characterized. With optimal conditions, the proposed probe P showed good selectivity to Mg2+ compared to other common metal ions, and worked in a wide linear range of 5.0 × 10(-7)-6.0 × 10(-6) M with a detection limit of 1.7 × 10(-7) M Mg2+ in ethanol-water solution (9:1, v/v, 20 mM HEPES, pH = 10.0).
متن کاملDetermination of mercury(II) in aquatic plants using quinoline-thiourea conjugates as a fluorescent probe.
In this study, a quinoline-thiourea conjugate (1-phenyl-3-(quinoline-8-yl) thiourea, PQT) was synthesized and used as a fluorescence sensor to detect mercury ion. The observation is coincident with the well-documented phenomenon that a thiocarbonyl-containing group on a fluorochrome quenches the fluorescence due to the heavy atom effect of the S atom. The large fluorescence enhancement of PQT i...
متن کاملIn-gel detection of biotin–protein conjugates with a green fluorescent streptavidin probe
Exploitation of the (strept)avidin–biotin interaction is extremely valuable in a variety of biotechnological applications. Biotin is often covalently linked to proteins or nucleic acids. Determination of the degree of biotinylation of such macromolecules is essential for downstream applications. There is currently a gap in simple yet efficient assays for rapidly quantitating protein biotinylati...
متن کاملSpecific tracking of xylan using fluorescent-tagged carbohydrate-binding module 15 as molecular probe.
BACKGROUND Xylan has been identified as a physical barrier which limits cellulose accessibility by covering the outer surface of fibers and interfibrillar space. Therefore, tracking xylan is a prerequisite for understanding and optimizing lignocellulosic biomass processes. RESULTS In this study, we developed a novel xylan tracking approach using a two-domain probe called OC15 which consists o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Canadian Journal of Chemistry
سال: 1981
ISSN: 0008-4042,1480-3291
DOI: 10.1139/v81-425