Synthesis of Cellulase during Abscission of Phaseolus vulgaris Leaf Explants
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چکیده
منابع مشابه
Synthesis of Cellulase during Abscission of Phaseolus vulgaris Leaf Explants.
When abscission in leaf explants from Phaseolus vulgaris, cultivar Red Kidney, was allowed to proceed while the explants were in (2)H(2)O, a 1.25% increase in the buoyant density of cellulase in a cesium chloride gradient was observed. These data indicate that the increase in cellulase activity during abscission is a result of the synthesis of new protein. Two differentially soluble forms of ce...
متن کاملCellulase and Abscission in the Red Kidney Bean (Phaseolus vulgaris).
Cellulase (beta-1, 4-glucan-glucanohydrolase EC 3.2.1.4) activity in the abscission zone of red kidney bean (Phaseolus vulgaris) was previously shown to exist in at least two different molecular forms. The form of the enzyme which has an isoelectric point of 4.5 is present in both abscising and nonabscising tissue and requires grinding for extraction. Another form of the enzyme which has an iso...
متن کاملActivity of pectin esterase and cellulase in the abscission zone of citrus leaf explants.
The activity of pectin esterase and cellulase in abscission of citrus explants was studied. No relation was established between pectin esterase and abscission, while cellulase activity was markedly increased before abscission and for a certain period after excision. IAA and cycloheximide delay abscission and cellulase activity, while ethylene and, to a lesser extent, GA(3) accelerate them. Appl...
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The solubility of cellulase extracted from the abscission zones of citrus leaf explants (Citrus sinensis L. Osbeck) in sodium phosphate buffer depends on the pH of the extracting solution and, to a lesser extent, on the ionic strength. By increasing molarity from 0.01 to 0.16, the solubility of cellulase increased from 51% to 89% at pH 6.1 and from 70% to 98% at pH 7. In all cases, residual cel...
متن کاملMethylammonium Transport in Phaseolus vulgaris Leaf Slices.
Methylammonium (as a nonmetabolized analog of ammonium) transport was studied in leaf slices of Phaseolus vulgaris L. var. ;Hawkesbury Wonder.' The relationship of influx to external pH (6.0-10.5) shows that the influx at low external pH is a larger fraction of that at high external pH than would be expected from the pK(alpha) of methylammonium and the assumption that only CH(3)NH(2) is enterin...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1970
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.46.2.194