Synthesis of calf prochymosin (prorennin) in Escherichia coli.
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منابع مشابه
Synthesis of calf prochymosin (prorennin) in Escherichia coli.
A gene for calf prochymosin (prorennin) has been reconstructed from chemically synthesized oligodeoxyribonucleotides and cloned DNA copies of preprochymosin mRNA. This gene has been inserted into a bacterial expression plasmid containing the Escherichia coli tryptophan promoter and a bacterial ribosome binding site. Induction of transcription from the tryptophan promoter results in prochymosin ...
متن کاملSolubilization and activation of recombinant calf prochymosin from Escherichia coli.
Lah, T., Turk, V. &Pain, R. H. (1984) Biochem. J. 218,601-608 McPhie, P. (1980) J. Biol. Chem. 255,4048-4052 Perlmann, G. E. (1956) Arch. Biochem. Biophys. 65,210-217 Privalov, P. L., Mateo, P. L., Khechinashvili, N. N., Stepanov, V. M. & Revina, L. P. (1981) J. Mol. Biol. 152,445-464 Schlamowitz, M., Peterson, L. V. & Wissler, F. C. (1961) Arch. Biochem. Biophys. 92,58-68 Shewale, J. G. & Tang...
متن کاملActivation studies of the multiple forms of prochymosin (prorennin).
Activation of the four separate components of prochymosin (prorennin) at pH 5.0 demonstrated that each zymogen was the precursor to an electrophoretically distinct chymosin (rennin). When the increase in milk-clotting activity with time was analysed, the mechanism of activation of unfractionated prochymosin, individual prochymosin components, and a mixture of the prochymosin fractions at pH 5.0...
متن کاملDenaturation studies on natural and recombinant bovine prochymosin (prorennin).
1. Prochymosin in solution in the presence of 8 M-urea is fully unfolded, as indicated by its fluorescence spectrum, fluorescence quenching behaviour and far-u.v.c.d. spectrum. 2. Equilibrium studies on the unfolding of prochymosin and pepsinogen by urea were carried out at pH 7.5 and pH 9.0. The results indicate that the stabilization energies of the two proteins are identical at pH 7.5, but t...
متن کاملClonal Relatedness of Enterotoxigenic and Enteropathogenic Escherichia coli Isolates from Diverse Human, Foods and Calf Sources
Background: Foodborne infection caused by EnterotoxigenicEscherichia coli (ETEC) and Enteropathogenic Escherichia coli (EPEC) is one of the major health problems, particularly in the developing countries. Therefore, it is vital to identify the origin of food contamination to plan control strategies efficiently. Method: A tota...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1983
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.80.12.3671