Synthesis of Branched Triubiquitin Active-Site Directed Probes
نویسندگان
چکیده
منابع مشابه
Active-site probes of flavoproteins.
The chemical reactivity of 8-chloroflavins and 8-mercaptoflavins has been exploited in order to examine the orientation of protein-bound flavins relative to solvent. The apoprotein form of a series of flavoproteins was prepared and the native flavin was replaced by either 8-C1-flavin or 8-mercaptoflavin (FAD, FMN, or riboflavin form as was appropriate). The reconstituted proteins were exposed t...
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Derivatives of phenyl-keto butenoic acids have been reported to be inhibitors of pyruvate decarboxylase, (PDC). The inhibition of transketolase, a thiamine requiring enzyme such as PDF, by meta nitrophenyl derivative of 2-oxo-3-butenoic acid (MNPB) is reported here. These studies indicate that the inhibitor binds to the enzyme at the active site. A two-step inhibition was observed, first th...
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BACKGROUND The proteasome is a multicatalytic protease complex responsible for most cytosolic protein breakdown. The complex has several distinct proteolytic activities that are defined by the preference of each for the carboxyterminal (P1) amino acid residue. Although mutational studies in yeast have begun to define substrate specificities of individual catalytically active beta subunits, litt...
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Each of the constituent enzymes of the pathway was present with sufficient activity to account for the observed rate of alginate biosynthesis. The information obtained from this work, together with other biochemical information, e.g. knowledge of the mechanisms of nitrogen fixation and respiration (Yates & Jones, 1974) and of the deposition of poly-8-hydroxybutyrate in A . vinelundii (Dawes & S...
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ژورنال
عنوان ژورنال: Organic Letters
سال: 2019
ISSN: 1523-7060,1523-7052
DOI: 10.1021/acs.orglett.9b02406