Synthesis of 2′‐Deoxy‐2′‐fluoro‐ <scp>L</scp> ‐cytidine and Fluorinated <scp>L</scp> ‐Nucleic Acids for Structural Studies

Structural and Biophysical Studies of Nucleic Acids

A Microfluidic Device for Structural Studies of Nucleic Acids

We assembled a microfluidic device that enables highresolution structural studies of nucleic acids. The device contains a mineral matrix capable of rapidly generating hydroxyl radicals to cleave the solvent accessible backbone of nucleic acids. Protection from radical cleavage (footprinting) can identify protein binding sites or the presence of tertiary structure. Here we report the fabrication...

Synthesis of Glycol Nucleic Acids Synthesis of Glycol Nucleic Acids

SYNTHESIS 2006, No. 4, pp 0645–0653xx.xx.2006 Advanced online publication: 25.01.2006 DOI: 10.1055/s-2006-926313; Art ID: M05505SS © Georg Thieme Verlag Stuttgart · New York Abstract: Starting from glycidol, the synthesis of dimethoxytritylated glycol nucleoside phosphoramidites of adenine (A), thymine (T), uracil (U), guanine (G), and cytosine (C) is reported. These phosphoramidites are the bu...

Monospecific antibody against 5-methyl-cytidine for the structural analysis of nucleic acids.

Anti 5-methyl-cytidine antibodies might be useful agents for the detection and localization of 5-methyl-cytidine of nucleic acids, but only if the antibodies recognize this nucleoside with sufficient specificity. A conjugate containing 18 moles of 5-methyl-cytidine per mole of BSA was prepared and antibodies directed against this nucleoside hapten were produced by immunization of rabbits (as de...

Synthesis and structural characterization of fluorinated thiosemicarbazones.

Six new fluorinated thiosemicarbazones R‑C(R')=N-NH-C(S)NH(2) (R = 2,4-C(6)H(3)F(2), R' = H (1); R = 2,5-C(6)H(3)F(2), R' = H (2); R = 2,6-C(6)H(3)F(2), R' = H (3); R = 3,4-C(6)H(3)F(2), R' = H (4); R = 3,5-C(6)H(3)F(2), R' = H (5) and R = 4-C(6)H(4)F, R' = C(6)H(5), (6)) have been prepared. The molecular structures of compounds 1 to 6 have been determined.