Synthesis and accumulation of amylase-trypsin inhibitors and changes in carbohydrate profile during grain development of bread wheat (Triticum aestivum L.)

Abstract Background Recent studies indicate that amylase-trypsin inhibitors (ATIs) and certain carbohydrates referred to as FODMAPs (fermentable oligo-, di-, monosaccharides polyols) play an important role in promoting wheat sensitivity. Hitherto, no study has investigated the accumulation of ATIs during development caryopsis. We collected caryopses common cv. ‘Arnold’ at eight different grain developmental stages compositional changes ATI FODMAP content. Results The harvested were analysed for their size, protein carbohydrate concentrations. further characterized by MALDI-TOF MS, trypsin inhibition was evaluated enzymatic assay. results showed started about 1 week after anthesis subsequently increased steadily until physiological maturity. However, biological activity terms enzyme not detectable before 4 weeks anthesis. Carbohydrate analysis revealed abundance short-chain fructans early development, whereas non-water-soluble later stages. Conclusions provide new insights into complex metabolisms filling maturation, with particular emphasis on content well inhibitory potential towards trypsin. time lag between is possibly attributed assembling dimers tetramers, which seems be crucial potential.