Synergism between coenzyme and alcohol binding to liver alcohol dehydrogenase

Coenzyme Interaction with Horse Liver Alcohol Dehydrogenase EVIDENCE FOR ALLOSTERIC COENZYME BINDING SITES FROM THERMODYNAMIC

The techniques of fluorescence enhancement, fluorescence quenching, fluorescence polarization, and equilibrium dialysis are utilized to study the binding properties of coenzyme to horse liver alcohol dehydrogenase. Polarization of fluorescence and equilibrium dialysis show that NADH binds to alcohol dehydrogenase with a stoichiometry of 6 mol per mol of enzyme, in contrast to the value of 2 det...

Coenzyme interaction with horse liver alcohol dehydrogenase. Evidence for allosteric coenzyme binding sites from thermodynamic equilibrium studies.

The techniques of fluorescence enhancement, fluorescence quenching, fluorescence polarization, and equilibrium dialysis are utilized to study the binding properties of coenzyme to horse liver alcohol dehydrogenase. Polarization of fluorescence and equilibrium dialysis show that NADH binds to alcohol dehydrogenase with a stoichiometry of 6 mol per mol of enzyme, in contrast to the value of 2 det...

Kinetic Studies of Liver Alcohol Dehydrogenase and pH with Coenzyme Preparations of High Purity Effects

Liver alcohol dehydrogenase has been the subject of repeated and detailed kinetic study. Because of its abundance, ready crystallization (1,2) and stability, the availability of pure, stable substrates, and the marked changes of absorption and fluorescence spectrum which accompany its combination with the reduced coenzyme (3), it has been to a considerable extent the immediate stimulus for the ...

Residual activity in carboxymethylated liver alcohol dehydrogenase.

The physiological role of liver alcohol dehydrogenase.

1. Yeast alcohol dehydrogenase was used to determine ethanol in the portal and hepatic veins and in the contents of the alimentary canal of rats given a diet free from ethanol. Measurable amounts of a substance behaving like ethanol were found. Its rate of interaction with yeast alcohol dehydrogenase and its volatility indicate that the substance measured was in fact ethanol. 2. The mean alcoho...