SYNERGIC EFFECT OF PREPARATION WITH COORDINATION COMPLEX “TRIMETHYDRAZINIUM PROPIONATE+ETHYMTH METHYLHYDROXYPIRIDINE SUCCINATE” ON ENERGY METABOLISM AND CELL RESPIRATION

The article presents the results of an in vitro study synergetic effect evaluation combined preparation based on coordination complex ethylmethylhydroxypyridine succinate and trimethylhydrazinium propionate energy metabolism cell respiration. aim was to evaluate mitochondria-directed action metabolic antioxidant succinic acid with relation optimizing cells under oxidative stress conditions, as well against background ischemic processes. Materials methods. hydroxysuccinate drug Brainmax® components carried out isolated mouse liver mitochondria. In course study, potential mitochondria, generation rate hydrogen peroxide during respiration, respiration were evaluated following positions: a) unstimulated by malate pyruvate, b) stimulated pyruvate (complex I substrates), II c) initial section electron transport chain blockade rotenone, d) phosphorylation oligomycin, e) FCCP-induced uncoupling, f) cyanide-blocked IV (cytochrome C oxidase). Results. It has been shown that trimethylhydrazinium, which is active principle drug, significantly reduced transmembrane mitochondria (IC 50 =197±5 µM), compared widely used preparations propionate, facilitates transfer produced ATP into preserves a vital activity even stress. mitochondrial substrates (NADP-coenzyme Q-oxidoreductase), malate, studied led more pronounced increase oxygen consumption IC =75±6 µМ. When evaluating production most observed addition complex, indicated uncoupling at given concentrations compounds. assessing significant decrease samples containing EMHPS. Conclusion. Based totality obtained, it can be assumed favorable conformation pharmacophore groups trimethylhydrozinium included composition leads interaction pharmacological effects target cells. This provides stabilization function, intensification adenosine triphosphate optimization processes cell, reduces severity eliminates undesirable ischemic-hypoxic tissue damage.