Symmetry breaking by enzyme-catalyzed epoxide hydrolysis
نویسندگان
چکیده
منابع مشابه
Laboratory‐Evolved Enzymes Provide Snapshots of the Development of Enantioconvergence in Enzyme‐Catalyzed Epoxide Hydrolysis
Engineered enzyme variants of potato epoxide hydrolase (StEH1) display varying degrees of enrichment of (2R)-3-phenylpropane-1,2-diol from racemic benzyloxirane. Curiously, the observed increase in the enantiomeric excess of the (R)-diol is not only a consequence of changes in enantioselectivity for the preferred epoxide enantiomer, but also to changes in the regioselectivity of the epoxide rin...
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Epoxides are highly useful synthons and biosynthons for the construction of complex natural products during total synthesis and biosynthesis, respectively. Among enzyme-catalyzed epoxide transformations, a reaction that is notably missing, in regard to the synthetic toolbox, is cationic rearrangement that takes place under strong acid. This is a challenging transformation for enzyme catalysis, ...
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The regioand enantioselective hydrolysis of several phenyloxiranes catalyzed by soluble epoxide hydrolase (sEH) was investigated using recombinant human, mouse or cress sEH. Results indicate that human and mouse sEH enantioselectively hydrolyze (S,S)-alkyl-phenyloxiranes faster than the (R,R)alkyl-phenyloxiranes investigated in this study, while cress sEH displayed opposite enantioselectivity. ...
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Enzyme efficiency was investigated in the lipase-catalyzed hydrolysis of canola oil in supercritical carbon dioxide (SCCO2). Immobilized lipase from Mucor miehie (Lipozyme IM) was used as the catalyst and the results showed that enzyme efficiency dropped at high pressures indicating a possible change in enzyme microstructure. Therefore, scanning electron microscopy (SEM) was used...
متن کاملSymmetry Breaking by Nonstationary Optimisation
We describe a new partial symmetry breaking method that can be used to break arbitrary variable/value symmetries in combination with depth first search, static value ordering and dynamic variable ordering. The main novelty of the method is a new dominance detection technique based on local search in the symmetry group. It has very low time and memory requirements, yet in preliminary experiments...
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ژورنال
عنوان ژورنال: IUCrJ
سال: 2018
ISSN: 2052-2525
DOI: 10.1107/s2052252518007005