Sustainable Coated Nanostructures Based on Alginate and Electrospun Collagen Loaded with Antimicrobial Agents

In this study, sodium alginate film (Alg) was coated with electrospun collagen glue (Col) extracted from rabbit skin waste, loaded different commercial antimicrobial agents (chitosan, AG425K and ZnONPs) investigated in terms of morphological, structural biological properties. The nanostructures were characterized using scanning electron microscopy coupled the energy-dispersive X-ray (SEM/EDS), Attenuated Total Reflectance Fourier-Transform Infrared spectroscopy (ATR FT-IR), Atomic Force Microscopy (AFM) tests. cytotoxicity on murine L929 fibroblasts 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide salt (MTT) lactate dehydrogenase (LDH) assays. Microbiological tests performed against Staphylococcus aureus ATCC 25923, Escherichia coli 25922, Pseudomonas aeruginosa 27853 Candida albicans standard strains. vitro cell culture showed a good cytocompatibility nanostructured systems, except sample ZnONPs, which exhibited highly cytotoxic effect. Alg-Col-ZnONPs nanostructure inhibited growth multiplication 25923 25922 bacterial results new may be useful for development sustainable biomaterials circular economy, bioactive properties medical wound dressings.