Susceptibility of polar cod (Boreogadus saida) to a model carcinogen

Studies that aim to characterise the susceptibility of ecologically relevant and non-model fish polar cod (Boreogadus saida) model carcinogens are required. Polar were exposed under laboratory conditions for six months control, 0.03 μg BaP/g fish/week 0.3 dietary benzo(a)pyrene (BaP), a reference carcinogen. The concentrations 3-OH-BaP bile metabolite transcriptional responses genes involved in DNA adduct recognition (xpc), helicase activity (xpd), repair (xpf, rad51) tumour suppression (tp53) assessed after 0, 1, 3 6 exposure, alongside body condition indexes (gonadosomatic index, hepatosomatic index factor). Micronuclei nuclear abnormalities blood spleen, liver histopathological endpoints at end experiment. Fish grew steadily over whole experiment no mortality was recorded. increased significantly 1 month exposure highest BaP concentration all showing biotransformation mother compound. Nevertheless, significant induction gene transcripts damage or observed selected sampling times. These results together with absence chromosomal spleen cells, subtle increase cells low occurrence foci cellular alteration suggested below threshold observable effects. Taken together, showed not susceptible carcinogenesis using regime employed herein.