Survival Rates of Rapidly Frozen Bovine Spermatozoa
نویسندگان
چکیده
منابع مشابه
Reaction of fresh and frozen bull spermatozoa incubated with fresh and frozen bovine oviduct epithelial cells.
The hypothesis was tested that frozen bovine oviduct epithelial cells (BOEC) could provide a physiologic and standardized system for studying motility and acrosomal changes of fresh and frozen-thawed sperm. The BOEC were collected by lavage from cows and monolayers prepared from both fresh and frozen-thawed cells. Fresh and frozen semen processed from five bulls was extended in a modified Tyrod...
متن کاملEffect of rates of freezing, thawing and level of glycerol on the survival of bovine spermatozoa in straws.
Extended semen from six Hereford bulls was placed in .25-ml Continental straws and frozen in the vertical position. Treatments were arranged factorially with glycerol levels of 5, 7, 9 or 11%; semen cooled from 5 to 1 3 0 C in 3.5 (fast), 20 (moderate) or 40 min (slow); and thawed in water at 5 C for 3 min, 35 C for 12 sec, 55 C for 8 sec, 75 C for 7 sec or 90 C for 5 seconds. Fast freezing res...
متن کاملEffect of thawing procedures on fertility of bovine spermatozoa frozen in .25-ml straws.
The influence of thawing procedures on fertility of semen in .25-ml Continental 3 straws was investigated in a series of eight trials utilizing 1,634 cows. The following pregnancy rates were obtained: Trial 1, 38.6 vs 23.0% (P<.05) for semen thawed in 75 C water/7 sec vs "in the cow"; Trial 2, 29.7 vs 22.2% (P>.10) for semen thawed in 75 C water/7 sec vs "in the cow"; Trial 3, 82.8 vs 61.6% (P<...
متن کاملFrozen boar spermatozoa: methods of thawing pellets.
In four experiments, the effects of pre-thaw delay, method of thawing, temperature of thawing solution and pellet volume on acrosome morphology and moti l i ty of" boar spermatozoa frozen by the pellet method were examined. Holding the frozen pellets for 0, 1.5, 3 or 4.5 min in a Styrofoam container before transferring them into the thawing solution resulted in maximum post-thaw percentages of ...
متن کاملStructure of rapidly frozen gap junctions
The structure of gap junctions in the rabbit ciliary epithelium, corneal endothelium, and mouse stomach and liver was studied with the freeze-fracturing technique after rapid freezing to near 4 degrees K from the living state. In the ciliary epithelium, the connexons were randomly distributed, separated by smooth membrane matrix. In the corneal endothelium, both random and crystalline arrangeme...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1960
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(60)90294-0