Survival of tubercle bacilli in heat-fixed and stained sputum smears
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چکیده
منابع مشابه
Survival of tubercle bacilli in heat-fixed sputum smears.
Tubercle bacilli, which survived heat fixation, were detected with a slide culture technique which allowed the entire smear to be examined. Both conventional flame fixation and the use of a controlled hot-plate failed to render tuberculous sputum smears safe for further handling. Smears which were stained with the phenol-auramine method failed to yield growth on culture. If delay between prepa...
متن کاملSurvival of tubercle bacilli in heat-fixed and stained sputum smears.
We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in...
متن کاملExamination of smears for tubercle bacilli by fluorescence microscopy.
I n underdeveloped countries, laboratory faci l i ties for the bacterio logical d iagnosis of tubercu losis are, at present , very l imited . Cultural methods are un l i kely to be used on a large scale for many years to come. It is , therefore, important to investigate the most economical method of examin ing smears for tubercle baci l l i . Fluorescence microscopy was introduced by H agemann...
متن کاملRelationship between CT Findings of Pulmonary Tuberculosis and Acid-Fast Bacilli Sputum Smears
Background and purpose: Computed tomography (CT) scan is of great help in confirming the diagnosis of tuberculosis (TB) before the sputum test result. The purpose of this study was to investigate the relationship between pulmonary involvement patterns in CT scans of TB patients with positive sputum smears. Materials and methods: This retrospective descriptive-analytical study was conducted usi...
متن کاملAutomated identification of tubercle bacilli in sputum. A preliminary investigation.
OBJECTIVE To use an automated method to detect tubercle bacilli in sputum specimens. STUDY DESIGN Using fluorescence microscopy, tubercle bacilli were identified on auramine-stained sputum specimens. Images were then captured with a digital camera and enhanced through imaging processing techniques. The bacilli were recognized using neural network classifiers. RESULTS This preliminary invest...
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ژورنال
عنوان ژورنال: Memórias do Instituto Oswaldo Cruz
سال: 2001
ISSN: 0074-0276
DOI: 10.1590/s0074-02762001000200024