Supramolecular Control over Split-Luciferase Complementation
نویسندگان
چکیده
منابع مشابه
Supramolecular Control over Split‐Luciferase Complementation
Supramolecular split-enzyme complementation restores enzymatic activity and allows for on-off switching. Split-luciferase fragment pairs were provided with an N-terminal FGG sequence and screened for complementation through host-guest binding to cucurbit[8]uril (Q8). Split-luciferase heterocomplex formation was induced in a Q8 concentration dependent manner, resulting in a 20-fold upregulation ...
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Bioluminescent proteins such as luciferases are unique analytical tools with high sensitivity and wide dynamic ranges. However, applicability of the proteins has remained limited to reporter gene analysis. Split luciferase complementation technique is a new powerful strategy to detect protein-protein interactions and their related intracellular signaling in living cells. This review presents a ...
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ژورنال
عنوان ژورنال: Angewandte Chemie International Edition
سال: 2016
ISSN: 1433-7851
DOI: 10.1002/anie.201602807