Supported Bilayer Electrophoresis under Controlled Buffer Conditions
نویسندگان
چکیده
منابع مشابه
Separation of membrane-bound compounds by solid-supported bilayer electrophoresis.
Separation, purification, and detection of biomembrane species such as lipids and transmembrane proteins are difficult tasks. The processing conditions are often harsh, which can result in alteration of native structures or complete loss of material.1,2 Furthermore, it is difficult to detect subtle post-translational changes in these molecules that occur on the cell surface.3-5 Typical purifica...
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Supported lipid bilayers (SLB) are important for the study of membrane-based phenomena and as coatings for biosensors. Nevertheless, there is a fundamental lack of understanding of the process by which they form from vesicles in solution. We report insights into the mechanism of SLB formation by vesicle adsorption using temperature-controlled time-resolved fluorescence microscopy at low vesicle...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2011
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac1028819