Supercoiling-dependent flexibility of adenosine-tract-containing DNA detected by a topological method
نویسندگان
چکیده
منابع مشابه
DNA Spiral Supercoiling and Intramolecular Topological Interlink
Escherichia coli chromosome DNA was observed previously to consist of subunits of Archimedean spirallike supercoiling. How do cells build such a DNA structure remains unknown. In the present study, atomic force microscopy (AFM) images showed that supercoiled pBR322 DNA forms a spiral structure upon intercalation with 0.5 μg/ml ethidium bromide (EB), which used to be considered as zero superheli...
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Inhibition of DNA supercoiling-dependent transcriptional activation by a distant B-DNA to Z-DNA transition.
Negative DNA superhelicity can destabilize the local B-form DNA structure and can drive transitions to other conformations at susceptible sites. In a molecule containing multiple susceptible sites, superhelicity can couple these alternatives together, causing them to compete. In principle, these superhelically driven local structural transitions can be either facilitated or inhibited by protein...
متن کاملNucleotide- and stoichiometry-dependent DNA supercoiling by reverse gyrase.
Reverse gyrase is a unique type IA topoisomerase that can introduce positive supercoils into DNA. We have investigated some of the biochemical properties of Archaeoglobus fulgidus reverse gyrase. It can mediate three distinct supercoiling reactions depending on the adenine nucleotide cofactor that is present in the reaction. Besides the ATP-driven positive supercoiling reaction, the enzyme can ...
متن کاملEffects of DNA supercoiling on the topological properties of nucleosomes.
In the nucleosome core particle, at least 145 base pairs of DNA are bound to the histone octamer in a superhelical conformation. We have asked what effect the presence of these particles has on the ability of DNA gyrase to supercoil DNA. Synthetic minichromosomes, constructed by reconstituting complexes of core histones with the closed circular plasmid pBR322, were treated with various amounts ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1997
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.94.7.2817