SULFHYDRYL AND PROSTHETIC GROUPS OF LACTIC OXIDATIVE DECARBOXYLASE FROM MYCOBACTERIUM PHLEI

Mechanism of action and crystallization of lactic oxidative decarboxylase from Mycobacterium phlei.

Earlier phases of an investigation concerning the lactic oxidative decarboxylase’ from Mycobacterium phlei (1, 2) extended the initial observations of Edson (3) and Edson and Cousins (4). A similar type of enzyme, obtained from Mycobacterium avium, has been described by Yamamura et al. (5, 6). The flavoprotein character of the enzyme, as well as the absence of associated coenzymes, has been est...

Isolation from Mycobacterium Phlei

Mycobactin is the name suggested for a growth factor, present in acid-fast bacteria, which is essential for the growth of Mycobacterium johnei. A preliminary account ofthe work reported here has already been published (Francis, Madinaveitia, Macturk & Snow, 1949). Myco. johnei is an organism first observed by Johne & Frothingham (1895) in cases of chronic enteritis of cattle. It could not be cu...

The effect of sulfhydryl inhibitors on substrate oxidation and proline transport with membrane preparations from Mycobacterium phlei.

Substrate oxidation and amino acid transport were examined in the presence of sulfhydryl reagents with membrane preparations from Mycobacferium phlei. Oxidation of exogenously added @-NADH, succinate, ascorbate N, N, N’, IV’-tetramethyl-p-phenylenediamine, or ascorbate phenazinc methosulfate was inhibited by organic mercurials. The organic mercurials inhibited succinate oxidation between cytoch...

Genetic transfer in Mycobacterium phlei.

Purification and characterization of dihydrofolate reductase from Mycobacterium phlei.

The dihydrofolate reductase from Mycobacterium phlei was purified and characterized; it has an Mr of 15 000 and a pI of 4.8. It is competitively inhibited by both methotrexate and trimethoprim, although the affinity is less than for other bacterial dihydrofolate reductases.