Sugar-Binding Activity of Pea Lectin Expressed in White Clover Hairy Roots
نویسندگان
چکیده
منابع مشابه
Sugar-binding activity of pea lectin enhances heterologous infection of transgenic alfalfa plants by Rhizobium leguminosarum biovar viciae.
Transgenic alfalfa (Medicago sativa L. cv Regen) roots carrying genes encoding soybean lectin or pea (Pisum sativum) seed lectin (PSL) were inoculated with Bradyrhizobium japonicum or Rhizobium leguminosarum bv viciae, respectively, and their responses were compared with those of comparably inoculated control plants. We found that nodule-like structures formed on alfalfa roots only when the rhi...
متن کاملFlavonoids exclusively present in mycorrhizal roots of white clover exhibit a different effect on arbuscular mycorrhizal fungi than flavonoids exclusively present in non-mycorrhizal roots of white clover
The flavonoids 5,6,7,8,9-hydroxy chalcone, 3,7-hydroxy-4?methoxy flavone, 5,6,7,8-hydroxy-4?-methoxy flavone and 3,5,6,7,4?-hydroxy flavone can be detected only in non-mycorrhizal roots of white clover, but not in mycorrhizal roots, whereas the flavonoids acacetin, quercetin and rhamnetin are only present in mycorrhizal roots. We tested the effect of several concentrations of these compounds on...
متن کاملThermodynamics of monosaccharide binding to concanavalin A, pea (Pisum sativum) lectin, and lentil (Lens culinaris) lectin.
Titration calorimetry measurements of the binding of methyl alpha-D-mannopyranoside (Me alpha Man), D-mannopyranoside (Man), methyl alpha-D-glucopyranoside (Me alpha Glu), and D-glucopyranoside (Glu) to concanavalin A (Con A), pea lectin, and lentil lectin were performed at 281 and 292 K in 0.01 M dimethylglutaric acid-NaOH buffer (pH 6.9) containing 0.15 M NaCl and Mn+2 and Ca+2 ions. The site...
متن کاملRhizobium attachment to clover roots.
The adhesion of rhizobia to surfaces of clover roots was examined by an indirect plate-counting assay and phase-contrast microscopy. The number of Rhizobium trifolii cells attached to clover root segments increased in approximately linear fashion during the first hour of incubation, but did not change appreciably thereafter. The addition of 30 mM-2-deoxy-D-glucose, which effectively inhibits bi...
متن کاملdistribution of 32p between roots and tops of white clover: effect of mycorrhizal fungi and placement distance from the roots
in a greenhouse experiment, the effects of glomus intraradices and indigenous mycorrhizal isolates from soils under plow and no-till treatments on the uptake of 32p placed at 1, 2.5 and 4 cm from the roots of white clover (trifolium repens) in 23, 37 or 46 days after planting were compared.spores of the indigenous fungi were mostly a mixture of glomus mossea, g. clarum, g. caledonium and g....
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1995
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.109.4.1167