Substrate and Enzyme Characteristics that Limit Cellulose Hydrolysis
نویسندگان
چکیده
منابع مشابه
Substrate and Enzyme Characteristics that Limit Cellulose Hydrolysis.
The ability and, consequently, the limitations of various microbial enzyme systems to completely hydrolyze the structural polysaccharides of plant cell walls has been the focus of an enormous amount of research over the years. As more and more of these extracellular enzymatic systems are being identified and characterized, clear similarities and differences are being elucidated. Although much h...
متن کاملIncreased enzyme binding to substrate is not necessary for more efficient cellulose hydrolysis.
Substrate binding is typically one of the rate-limiting steps preceding enzyme catalytic action during homogeneous reactions. However, interfacial-based enzyme catalysis on insoluble crystalline substrates, like cellulose, has additional bottlenecks of individual biopolymer chain decrystallization from the substrate interface followed by its processive depolymerization to soluble sugars. This a...
متن کاملCellulose hydrolysis in evolving substrate morphologies III: time-scale analysis.
We present a time-scale analysis for the enzymatic hydrolysis of solid cellulosic substrates, based on our recently developed kinetic model (Zhou et al., 2009a, Biotechnol Bioeng 104:261-274; Zhou et al., 2009b, Biotechnol Bioeng 104:275-289) which incorporates both enzymatic chain fragmentation and hydrolytic time evolution of the solid substrate morphology. Analytical order-of-magnitude estim...
متن کاملEnzyme-microbe synergy during cellulose hydrolysis by Clostridium thermocellum.
Specific cellulose hydrolysis rates (g of cellulose/g of cellulase per h) were shown to be substantially higher (2.7- to 4.7-fold) for growing cultures of Clostridium thermocellum as compared with purified cellulase preparations from this organism in controlled experiments involving both batch and continuous cultures. This "enzyme-microbe synergy" requires the presence of metabolically active c...
متن کاملThe effect of agitation speed, enzyme loading and substrate concentration on enzymatic hydrolysis of cellulose from brewer’s spent grain
Brewer’s spent grain components (cellulose, hemicellulose and lignin) were fractionated in a two-step chemical pretreatment process using dilute sulfuric acid and sodium hydroxide solutions. The cellulose pulp produced was hydrolyzed with a cellulolytic complex, Celluclast 1.5 L, at 45 C to convert the cellulose into glucose. Several conditions were examined: agitation speed (100, 150 and 200 r...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biotechnology Progress
سال: 1999
ISSN: 8756-7938
DOI: 10.1021/bp9900864