Subspecies typing of Vibrio parahaemolyticus by pulsed-field gel electrophoresis
نویسندگان
چکیده
منابع مشابه
Subspecies typing of Vibrio parahaemolyticus by pulsed-field gel electrophoresis.
Vibrio parahaemolyticus is one of the most important food-borne pathogens in Taiwan, Japan, and other costal regions. We report on the development of a pulsed-field gel electrophoresis (PFGE) method for the molecular typing of this pathogen. Genomic DNA was digested with SfiI, and the fragments were resolved on 1% agarose with a contour-clamped homogeneous electric field apparatus set at 190 V ...
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This study reports a characteristic of some Vibrio parahaemolyticus strains which are susceptible to electrophoresis-related DNA-degradation (ERDD) by pulsed-field gel electrophoresis (PFGE) in Tris-borate EDTA (TBE) buffer. V. parahaemolyticus is a halophilic bacterium naturally inhabiting coastal environments worldwide, with reported pandemic strains, warranting the need for surveillance and ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1996
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.34.6.1535-1539.1996