Submonomer synthesis of sequence defined peptoids with diverse side-chains

Peptoids are a diverse family of sequence-defined oligomers N-substituted glycine monomers, that can be readily accessed by the solid-phase submonomer synthesis method. Due to versatility and efficiency this chemistry, easy access hundreds potential there is an enormous sequence space explored. This has enabled researchers from many different fields custom-design peptoid sequences tailored wide variety problems in biomedicine, nanoscience polymer science. Here we provide detailed protocols for peptoids, using optimized performed non-chemists. The method fully compatible with Fmoc-peptide conditions, so automated on existing peptide synthesizers provided here. Although peptoids well established, special considerations required order most useful desirable sidechains. methods include amino-acid-like side chains, some important non-natural monomer classes, as creation conjugates peptide-peptoid hybrids.