Subcellular fractionation of anaerobically grown Saccharomyces carlsbergensis by zonal centrifugation
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چکیده
منابع مشابه
Subcellular fractionation by differential and zonal centrifugation of aerobically grown glucose-de-repressed Saccharomyces carlsbergensis.
1. Homogenates were prepared from sphaeroplasts of aerobically grown glucose-de-repressed Saccharomyces carlsbergensis and the distributions of marker enzymes were investigated after differential centrifugation. Cytochrome c oxidase and cytochrome c were sedimented almost completely at 10(5)g-min, and this fraction also contained 37% of the catalase, 27% of the acid p-nitrophenyl phosphatase, 5...
متن کاملSubcellular fractionation of particles containing acid hydrolases from Saccharomyces carlsbergensis.
It has been shown that, under suitable conditions, the lysis of osmotically stabilized sphaeroplasts of yeast leads to the release of cell-vacuoles, which can then be purified on iso-osmotic gradients (Matile & Wiemken, 1967). On the basis of these fractionations these authors claim that the single large cell-vacuole is the only lysosome of the yeast cell. However, it has been shown that, by us...
متن کاملFractionation of liver plasma membranes prepared by zonal centrifugation.
1. Plasma membranes were isolated from crude nuclear sediments from mouse and rat liver by a rate-dependent centrifugation through a sucrose density gradient contained in the ;A' type zonal rotor. 2. The membranes were further purified by isopycnic centrifugation, and characterized enzymically, chemically and morphologically. 3. When the plasma-membrane fraction of sucrose density 1.17g/cm(3) w...
متن کاملThe fractionation of nuclei from mammalian cells by zonal centrifugation.
1. Purified liver nuclei from adult rats separate into two main zones when centrifuged in the slow-speed zonal rotor. One zone contains diploid nuclei, the other tetraploid. 2. The effect of age on the pattern of rat liver ploidy was examined. Tetraploid nuclei are virtually absent from young animals. They increase in proportion steadily with age. Partial hepatectomy disturbs the pattern of plo...
متن کاملA protocol for the subcellular fractionation of Saccharomyces cerevisiae using nitrogen cavitation and density gradient centrifugation
Most protocols for yeast subcellular fractionation involve the use of mechanical shear forces to lyse the spheroplasts produced by the enzymatic digestion of the Saccharomyces cerevisiae cell wall. These mechanical homogenization procedures often involve the manual use of devices such as the Dounce homogenizer, and so are very operator-dependent and, in consequence, lack reproducibility. Here, ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1970
ISSN: 0306-3283
DOI: 10.1042/bj1160024p