Study the Role of the Efflux Pump in Multidrug-Resistant Acinetobacter baumannii

Thirty-one A. baumannii isolates were obtained from Shar and Burn hospitals in Sulaimani City, KRG-Iraq. Bacterial identification was confirmed by detecting both 16S rRNA blaOXA-51 genes. The detection of carbapenem resistant carried out using Kirby- Bauer, RAPDEC Carba NP combined disc assays. A high rate drug resistance observed, as 74.2% the multidrug-resistant (MDR) 38.7% extensively drug-resistant (XDR), while carbapenem-resistant (CRAB) comprised approximately 58% isolates. Among CRAB isolates, carbapenemase production detected assays 88.8% (16/18) 55.5% (10/18) respectively. Phenotypic activity efflux pump among 22.5% all 38.8% PCR successfully identified adeA, adeB, adeC, adeR, adeS genes 83.9%, 90.3%, 58%, 80.6%, 90.3% respectively.
 Eight mutations adeR 15 regulatory recorded. We conclude that mechanisms bumanni included adeRS carbapenemase.